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Programmed Cell Death 1 (PDCD1) (AA 25-167) (Active) protein (His tag,AVI tag,Biotin) Protein

PDCD1 Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Pubmed (4 Referenzen)
Produktnummer ABIN2181593
Zzgl. Versandkosten $45.00
Lieferung in 2 bis 3 Werktagen
  • Target Alle PD-1 (PDCD1) Proteine anzeigen
    PD-1 (PDCD1)
    Biologische Aktivität
    AA 25-167
    • 27
    • 11
    • 5
    • 4
    • 4
    • 1
    • 1
    • 34
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses PD-1 Protein ist gelabelt mit His tag,AVI tag,Biotin.
    AA 25-167
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 18.6 kDa. The protein migrates as 30-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    >95 % as determined by SDS-PAGE.
    0.22 μm filtered
    Less than 1.0 EU per μg by the LAL method.
  • Kommentare

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Nur für Forschungszwecke einsetzbar
  • Format
    PBS, pH 7.4
    Please avoid repeated freeze-thaw cycles.
    -20 °C
    Informationen zur Lagerung
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • Wang, Fei, Jing, Wu, Wu, Zhou, Ni, Chen, Xiong, Liu, Peng, Yu, Jiang, Liu: "Durable blockade of PD-1 signaling links preclinical efficacy of sintilimab to its clinical benefit." in: mAbs, (2019) (PubMed).

    Ganesan, Ahmed, Okoye, Arutyunova, Babu, Turnbull, Kundu, Shields, Agopsowicz, Xu, Tabana, Srivastava, Zhang, Moon, Belovodskiy, Hena, Kandadai, Hosseini, Hitt, Walker, Smylie, West, Siraki, Lemieux et al.: "Comprehensive in vitro characterization of PD-L1 small molecule inhibitors. ..." in: Scientific reports, Vol. 9, Issue 1, pp. 12392, (2019) (PubMed).

    Son, Park, Seo, Lee, Heo, Kim: "A small-sized protein binder specific for human PD-1 effectively suppresses the tumour growth in tumour mouse model." in: Journal of drug targeting, pp. 1-9, (2019) (PubMed).

    Fromm, de Silva, Johannes, Patel, Hornblower, Schreiber: "Agonist redirected checkpoint, PD1-Fc-OX40L, for cancer immunotherapy." in: Journal for immunotherapy of cancer, Vol. 6, Issue 1, pp. 149, (2018) (PubMed).

  • Target
    PD-1 (PDCD1)
    Andere Bezeichnung
    PD-1 (PDCD1 Produkte)
    CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, Ly101, Pdc1, programmed cell death 1, PDCD1, Pdcd1
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
    18.6 kDa
    NCBI Accession
    Cancer Immune Checkpoints
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