SIRPA Protein (AA 31-370) (His tag)

Produktnummer ABIN2180706

Produktdetails

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 31-370

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SIRPA Protein ist gelabelt mit His tag.

Sequenz: AA 31-370

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.1 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Güteklasse: HPLC verified

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

Referenzen

Yu, Qiu, Long, Yang, Zhang, Xu, Chang, Du, Hou, Yu, Zeng, Wang, Sun: "A novel fully human anti-CD47 antibody as a potential therapy for human neoplasms with good safety." in: Biochimie, Vol. 151, pp. 54-66, (2018) (PubMed).

Antigendetails

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Andere Bezeichnung: SIRP alpha (SIRPA Produkte)

Hintergrund: Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Molekulargewicht: 38.5 kDa

NCBI Accession: NP_001035111