FGF2 Protein

Produktnummer ABIN2129216

Produktdetails

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Sequenz: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Produktmerkmale: Purified recombinant Human FGF basic protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is

Reinheit: > 98 % pure

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Kommentare:

Biological activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/mL, corresponding to a specific activity of 2,000,000 Units/mg.

Testdurchführung:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in distilled water to at least 100 μg/mL.

Buffer: Supplied in lyophilized form containing 10 mM Acetic acid,

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Referenzen

Czekanska, Ralphs, Alini, Stoddart: "Enhancing inflammatory and chemotactic signals to regulate bone regeneration." in: European cells & materials, Vol. 28, pp. 320-34, (2014) (PubMed).

Antigendetails

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: FGF Basic (FGF2 Produkte)

Synonyme: BFGF Protein, FGF-2 Protein, FGFB Protein, HBGF-2 Protein, Fgf-2 Protein, Fgfb Protein, bFGF Protein, fibroblast growth factor 2 Protein, fibroblast growth factor 2 (basic) Protein, FGF2 Protein, Fgf2 Protein, fgf2 Protein

Hintergrund: FGF basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Alternative Names: FGF-2 protein, HBGH-2 protein, HBGF-2 protein, FGF-b protein, Fibroblast Growth Factor Basic protein, Prostatropin protein

Molekulargewicht: 17,353 Da

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine