Cathepsin G Protein (CTSG)
(1 Validierung)-
- Target Alle Cathepsin G (CTSG) Proteine anzeigen
- Cathepsin G (CTSG)
- Protein-Typ
- Native
-
Spezies
- Human
-
Quelle
- Human
- Reinheit
- > 96 % pure
-
Cathepsin G (CTSG) (AA 146-255) protein (GST tag)
CTSG Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Cathepsin G (CTSG) (AA 1-255) protein (GST tag)CTSG Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Cathepsin G (CTSG) (AA 21-255) protein (His tag)Crystallography grade CTSG Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Cathepsin G (CTSG) (AA 21-261) protein (His tag)Crystallography grade CTSG Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Cathepsin G (CTSG) proteinCTSG Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
Cathepsin G (CTSG) (AA 21-261) protein (His-SUMO Tag)CTSG Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
-
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- by
- NanoTemper Technologies
- No.
- #102720
- Datum
- 05.11.2018
- Antigen
- CTSG
- Chargennummer
- A14041801
- Validierte Anwendung
- Unfolding Profile
- Positivkontrolle
- ABIN2128355
- Negativkontrolle
- Bewertung
Passed. ABIN2128355 showed a Ti of 63.8 °C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.
- Primärantikörper
- Sekundärantikörper
- Full Protocol
- Dilute ABIN2128355 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.5µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Anmerkungen
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
Validierung #102720 (Unfolding Profile)!['Independent Validation' Siegel]( "Erfolgreich validiert")
!['Independent Validation' Siegel]( "Erfolgreich validiert")
Validierungsbilder![Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 63.8°C.]()
Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 63.8°C.
Protokoll -
- Format
- Liquid
- Konzentration
- 250 μg/mL
- Buffer
- Liquid 50 mM sodium acetate, pH 5.5, 0.8 M sodium chloride
- Lagerung
- 4 °C/-20 °C/-80 °C
-
- Target
- Cathepsin G (CTSG)
- Andere Bezeichnung
- Cathepsin G (CTSG Produkte)
- Synonyme
- CATG Protein, CG Protein, CTSG Protein, Ctsg Protein, cathepsin G Protein, CTSG Protein, Ctsg Protein, LOC505658 Protein, LOC509956 Protein, ctsg Protein, LOC100053921 Protein, LOC102174648 Protein, LOC100733619 Protein, LOC100154047 Protein
- Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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