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Cathepsin G (CTSG) Protein Protein

CTSG Spezies: Human Quelle: Human Native > 96 % pure
Independent Validation (1)
Produktnummer ABIN2128355
$424.46
Zzgl. Versandkosten $45.00
1 mg
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 9 bis 11 Werktagen
  • Proteinname
    Protein-Typ
    Native
    Spezies
    Human
    Quelle
    Human
    Reinheit
    > 96 % pure
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #102720 (Unfolding Profile)
    'Independent Validation' Siegel
    by
    NanoTemper Technologies
    No.
    #102720
    Datum
    05.11.2018
    Antigen
    CTSG
    Chargennummer
    A14041801
    Validierte Anwendung
    Unfolding Profile
    Positivkontrolle
    ABIN2128355
    Negativkontrolle
    Bewertung

    Passed. ABIN2128355 showed a Ti of 63.8 °C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    Sekundärantikörper
    Full Protocol
    • Dilute ABIN2128355 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.5µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Anmerkungen

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Liquid
    Konzentration
    250 μg/mL
    Buffer
    Liquid 50 mM sodium acetate, pH 5.5, 0.8 M sodium chloride
    Lagerung
    4 °C/-20 °C/-80 °C
  • Proteinname
    Pathways
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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