Cathepsin G Protein (CTSG)

Produktnummer ABIN2128355

Produktdetails

Target: Cathepsin G (CTSG)

Protein-Typ: Native

Spezies: Human

Quelle: Human

Reinheit: > 96 % pure

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (UP)

Validierung #102720 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102720

Datum: 05.11.2018

Antigen: CTSG

Chargennummer: A14041801

Validierte Anwendung: Unfolding Profile

Positivkontrolle: ABIN2128355

Bewertung:

Passed. ABIN2128355 showed a T_i of 63.8 °C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Validierungsbilder

Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 63.8°C.

Protokoll

Full Protocol:

• Dilute ABIN2128355 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.5µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Anmerkungen:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Liquid 50 mM sodium acetate, pH 5.5, 0.8 M sodium chloride

Lagerung: 4 °C/-20 °C/-80 °C

Antigendetails

Target: Cathepsin G (CTSG)

Andere Bezeichnung: Cathepsin G (CTSG Produkte)

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones