Sonic Hedgehog Protein (SHH) (AA 25-198)

Produktnummer ABIN2115472

Dieses Recombinant Sonic Hedgehog-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Sonic Hedgehog Protein (SHH) (AA 25-198) (ABIN2115472)

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 25-198

Verwendungszweck: Recombinant Mouse Sonic Hedgehog/SHH

Sequenz: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG

Produktmerkmale: Recombinant Mouse Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys25-Gly198 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 weeks

Antigendetails

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Sonic Hedgehog/SHH

Hintergrund: Alternative Names: Sonic Hedgehog Protein, SHH, HHG-1, SHHUracil-DNA glycosylase(UNG)belongs to the uracil-DNA glycosylase family. The human gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Once unzipped, mismatched guanine and uracil pairs are separated, and DNA polymerase inserts complementary bases to form a guanine-cytosine (GC) pair in one daughter strand and an adenine-uracil (AU) pair in the other.Half of all progeny DNA derived from the mutated template inherit a shift from GC to AU at the mutation site.UDG excises uracil in both AU and GU pairs to prevent propagation of the base mismatch to downstream transcription and translation processes.
Synonyms: Uracil-DNA glycosylase,UDG,

Molekulargewicht: 19.8 kDa

UniProt: Q62226

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development