PDGF-BB Homodimer (AA 74-182) (Active) Protein

Produktnummer ABIN2018323

Recombinant -Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für PDGF-BB Homodimer (AA 74-182) (Active) Protein (ABIN2018323)

Target: PDGF-BB Homodimer

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE analysis.

Produktdetails

Proteineigenschaft: AA 74-182

Produktmerkmale: ED50 < 2 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5 x 10^5 units/mg.
AA 74-182, expressed with an N-terminal Met.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in 20 mM acetic acid at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 20 mM acetic acid.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: PDGF-BB Homodimer

Andere Bezeichnung: Platelet-Derived Growth Factor-BB (PDGF-BB)

Hintergrund: Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Platelet-Derived Growth Factor-BB, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

Molekulargewicht: 24.6 kDa, observed by non-reducing SDS-PAGE.

UniProt: Q05028