Interferon gamma Protein (IFNG)

Produktnummer ABIN2018005

Dieses Recombinant Interferon gamma-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Interferon gamma Protein (IFNG) (ABIN2018005)

Target: Interferon gamma (IFNG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as analyzed by reducing SDS-PAGE.

Produktdetails

Produktmerkmale: ED50<0.05 ng/mL, measured by cytotoxicity assay using HT-29 cells.
Gln24-Gln166 (accession number:P01579). Expression construct with an N-terminal Met.

Endotoxin-Niveau: < 1 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Interferon gamma (rhIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.

Haltbarkeit: 6 months

Antigendetails

Target: Interferon gamma (IFNG)

Andere Bezeichnung: Interferon-gamma (IFN-Gamma)

Hintergrund: Human Interferon gamma (hIFN-γ) is amacrophage-activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ-induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers. Recombinant human Interferon gamma (rhIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 144 amino acids. A fully biologically active molecule, rhIFN-γ has a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI,IFN-gamma.

Molekulargewicht: 17kDa, observed by reducing SDS-PAGE

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie