FGF8 Protein (AA 23-215)

Produktnummer ABIN2017830

Recombinant FGF8-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für FGF8 Protein (AA 23-215) (ABIN2017830)

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Produktdetails

Proteineigenschaft: AA 23-215

Produktmerkmale: ED50 < 5.0 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/mL of heparin, corresponding to a specific activity of > 2.0x 10^5 units/mg.
AA 23-215, expressed with an N-terminal Met.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Andere Bezeichnung: Fibroblast Growth Factor-8 (FGF-8)

Hintergrund: Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin- releasing hormone (GnRH) neuronal system.Recombinant human Fibroblast Growth Factor-8 (rhFGF-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 194 amino acids. A fully biologically active molecule, rhFGF-8 has a molecular mass of 22.5 kDa analyzed by reducing SDS-PAGE.
Synonyms: FGF-8b, AIGF, HBGF

Molekulargewicht: 22.5kDa, observed by reducing SDS-PAGE.

UniProt: P55075

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Dopaminergic Neurogenesis