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EIF4E Protein (AA 1-217)

Dieses Recombinant EIF4E-Protein wird in Escherichia coli (E. coli) exprimiert.
Produktnummer ABIN1945364

Kurzübersicht für EIF4E Protein (AA 1-217) (ABIN1945364)

Target

Alle EIF4E Proteine anzeigen
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein-Typ

Recombinant

Spezies

  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Quelle

  • 5
  • 5
  • 1
  • 1
Escherichia coli (E. coli)

Reinheit

> 95 % as determined by reducing SDS-PAGE.
  • Proteineigenschaft

    AA 1-217

    Verwendungszweck

    Recombinant Human Eukaryotic Translation Initiation Factor 4E/EIF4E

    Sequenz

    MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV

    Produktmerkmale

    Recombinant Human eIF-4E is produced by our E. coli expression system. The target protein is expressed with sequence (AA 1-217) of Human eIF-4E .

    Sterilität

    0.2 μm filtered

    Endotoxin-Niveau

    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

    Buffer

    Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

    Handhabung

    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

    Lagerung

    4 °C/-20 °C/-80 °C

    Informationen zur Lagerung

    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    Andere Bezeichnung

    Eukaryotic Translation Initiation Factor 4E

    Hintergrund

    Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

    Molekulargewicht

    25.1 kDa

    UniProt

    P06730

    Pathways

    BCR Signaling
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