GM-CSF Protein
Kurzübersicht für GM-CSF Protein (ABIN1589646)
Target
Alle GM-CSF (CSF2) Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Reinheit
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Verwendungszweck
- GM-CSF
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Sequenz
- APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
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Spezifität
- Chromosomal location:5q31.1
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Produktmerkmale
- Length (aa):127
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Endotoxin-Niveau
- < 0.1 ng per μg of GM-CSF
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Applikationshinweise
- The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.1 ng/mL corresponding to a specific activity of ≥1 x 10^7 units/mg.
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Kommentare
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Cytokines & Growth Factors
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- The lyophilized rh GM-CSF is soluble in water and most aqueous buffers and can be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
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Buffer
- PBS, pH 7.2
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Lagerung
- RT,-20 °C
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Informationen zur Lagerung
- The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted GM-CSF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Andere Bezeichnung
- GM-CSF
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Hintergrund
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Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific α chain and a common β chain that is shared by the high-affinity receptors for IL-3 and IL-5.
Synonyms: CSF2, GM-CSF -
Molekulargewicht
- 14.5 kDa
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Gen-ID
- 1437
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NCBI Accession
- NM_000758, NP_000749
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Pathways
- JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin
Target
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