Placenta Growth Factor 1 (PIGF-1) (Homodimer) (Active) Protein

Produktnummer ABIN1589556

Dieses Recombinant Placenta Growth Factor 1-Protein wird in Insektenzellen exprimiert.

Kurzübersicht für Placenta Growth Factor 1 (PIGF-1) (Homodimer) (Active) Protein (ABIN1589556)

Target: Placenta Growth Factor 1 (PIGF-1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Insektenzellen

Reinheit: > 95 % by SDS-PAGE

Produktdetails

Proteineigenschaft: Homodimer

Verwendungszweck: Recombinant Human PlGF-1 Protein

Sequenz: LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERCGDAVPR R

Spezifität: Chromosomal location:2p21-p16

Produktmerkmale: Length (aa):131

Anwendungsinformationen

Applikationshinweise: Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant human PlGF-1 can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5 - 10 ng/mL.

Kommentare:

Cytokines & Growth Factors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 mM acetic acid,Centrifuge vial prior to opening. The PlGF-1 is supplied in lyophilized form with carrier-protein (BSA) and can be reconstituted with 50 mM acetic acid or PBS/water. This solution can be diluted into other buffered solutions or stored frozen for future use.

Buffer: 50 mM acetic acid

Handhabung: Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: The lyophilized human PlGF-1, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: Placenta Growth Factor 1 (PIGF-1)

Hintergrund:

Synonyms: PlGF, placental growth factor

Description: Human Placenta Growth Factor-1 (PlGF-1), a 19 kDa protein consisting of 131 amino acid residues is produced as a homodimer. Human Placenta Growth Factor (PlGF) is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF-1 acts only as a very weak mitogen for some endothelial cell types and as a potent chemoattractant for monocytes. The physiological function in vivo is still controversal. In several reports it was shown not to be a potent mitogen for endotehlial cells and not angiogenic in vivo by using different assays. Very recently it was shown by one investigator, that PlGF-1 from cell culture supernatants was angiogenic in the CAM assay and in the rabbit cornea assay. At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes) but PlGF does not bind to KDR/flk-1. Two different proteins can be generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF-2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF-1 is a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms posses a similar biological profile. There is good evidence that heterodimeric molecules between VEGF and PlGF exists and that they are biological active. Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A very related protein of PlGF is VEGF with about 53 % homology and VEGF-B with similar biological activities.

Molekulargewicht: ~34.0 kDa

Gen-ID: 5228

NCBI Accession: NM_001207012, NP_001193941

UniProt: P49763