IL-2 Protein (AA 22-153)

Produktnummer ABIN1096921

Produktdetails

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 22-153

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Verwendungszweck: Recombinant Human Interleukin-2/IL-2

Sequenz: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT

Produktmerkmale: Recombinant Human Interleukin-2/IL-2 is produced by our E. coli expression system. The target protein is expressed with sequence (Pro22-Thr153) of Human IL-2.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Kommentare:

Biological activity: ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of murine CTLL-2 cells.Specific Activity of 1.0 x 107 IU/ mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Andere Bezeichnung: interleukin-2 (IL2 Produkte)

Synonyme: IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein

Hintergrund: Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated, 2) the molecule has no N-terminal alanine, 3) the molecule has serine substituted for cysteine at amino acid position 125, 4) the aggregation state of molecule is likely to be different from that of native IL-2.
Alternative Names: Interleukin-2, IL-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2

Molekulargewicht: 15.4 kDa

UniProt: P60568

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation