GM-CSF Protein (AA 18-144)

Produktnummer ABIN1096770

Produktdetails

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 18-144

Spezies: Human

Quelle: Yeast (Pichia pastoris)

Applikation: Functional Studies (Func)

Verwendungszweck: Recombinant Human GM-CSF/CSF2 (P. pastoris)

Sequenz: APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Produktmerkmale: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor/GM-CSF is produced with our Pichia pastoris expression system. The target protein is expressed with sequence (Ala18-Glu144) of Human GM-CSF.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Kommentare:

Biological activity: ED50 is less than 0.2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.Specific Activity of 5.0 x 106 IU/ mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 10 mM TrisHCl, 4 % Mannitol, 1 % Sucrose, pH 8.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: GM-CSF (CSF2 Produkte)

Hintergrund: GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages, and eosinophils, GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
Alternative Names: Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, Colony-Stimulating Factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF

Molekulargewicht: 14.4 kDa

UniProt: P04141

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin