GNS Protein (AA 37-552) (His tag)

Produktnummer ABIN1096744

Produktdetails

Target: GNS (Glucosamine (N-Acetyl)-6-Sulfatase (GNS))

Protein-Typ: Recombinant

Proteineigenschaft: AA 37-552

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses GNS Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human N-Acetylglucosamine-6-Sulfatase/GNS (C-6His)

Sequenz: VFGVAAGTRR PNVVLLLTDD QDEVLGGMTP LKKTKALIGE MGMTFSSAYV PSALCCPSRA SILTGKYPHN HHVVNNTLEG NCSSKSWQKI QEPNTFPAIL RSMCGYQTFF AGKYLNEYGA PDAGGLEHVP LGWSYWYALE KNSKYYNYTL SINGKARKHG ENYSVDYLTD VLANVSLDFL DYKSNFEPFF MMIATPAPHS PWTAAPQYQK AFQNVFAPRN KNFNIHGTNK HWLIRQAKTP MTNSSIQFLD NAFRKRWQTL LSVDDLVEKL VKRLEFTGEL NNTYIFYTSD NGYHTGQFSL PIDKRQLYEF DIKVPLLVRG PGIKPNQTSK MLVANIDLGP TILDIAGYDL NKTQMDGMSL LPILRGASNL TWRSDVLVEY QGEGRNVTDP TCPSLSPGVS QCFPDCVCED AYNNTYACVR TMSALWNLQY CEFDDQEVFV EVYNLTADPD QITNIAKTID PELLGKMNYR LMMLQSCSGP TCRTPGVFDP GYRFDPRLMF SNRGSVRTRR FSKHLLVDHH HHHH

Produktmerkmale:

Recombinant Human N-Acetylglucosamine-6-Sulfatase/GNS (C-6His)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl,10 % Glycerol, pH 8.0.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: GNS (Glucosamine (N-Acetyl)-6-Sulfatase (GNS))

Andere Bezeichnung: N-Acetylglucosamine-6-Sulfatase/GNS (GNS Produkte)

Hintergrund: Recombinant Human N-Acetylglucosamine-6-Sulfatase/GNS is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Val37-Leu552) of Human GNS fused with a polyhistidine tag at the C-terminus.
N-Acetylglucosamine-6-Sulfatase is a member of the Sulfatase family. N-Acetylglucosamine-6-Sulfatase is required for the lysosomal degradation of the Glycosaminoglycans (GAG) Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase hydrolyzes the 6-Sulfate groups of the N-Acetyl-D-Glucosamine 6-Sulfate units of Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase binds 1 Calcium ion per subunit. N-Acetylglucosamine-6-Sulfatase deficiency are the cause of Mucopolysaccharidosis Type 3D (MPS3D), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS3D has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.

Molekulargewicht: 59.35 kDa

UniProt: P15586

Pathways: Glycosaminoglycan Metabolic Process