C5A Protein (AA 679-755)

Produktnummer ABIN1096418

Dieses Recombinant C5A-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für C5A Protein (AA 679-755) (ABIN1096418)

Target: C5A (C5a) (Complement Component 5a (C5a))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 679-755

Verwendungszweck: Recombinant Mouse Complement Component C5

Sequenz: MNLHLLRQKI EEQAAKYKHS VPKKCCYDGA RVNFYETCEE RVARVTIGPL CIRAFNECCT IANKIRKESP HKPVQLGR

Produktmerkmale: Recombinant Mouse Complement C5a produced in E. coli is a single non-glycosylated polypeptide chain containing 78 amino acids (N679-R755) with a molecular mass of 9 kDa.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 350 mM NaCl, pH 7.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: C5A (C5a) (Complement Component 5a (C5a))

Andere Bezeichnung: c5a

Hintergrund: Mouse Complement C5 (C5a) is a glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 77 amino acid peptide that is created by the C5a convertase proteolytic cleavage of C5 alphachain in the classical and alternative complement pathway (C4b2a3b, C3bBb3b). Mouse C5a has fouralphahelices, plus three intra-chain disulfide bonds that form a triple loop structure. C5a functions via G-protein coupled receptor (GPCR) (C5aR/CD88). C5a is a potent chemoattractant and anaphylatoxin that acts on all classes of leukocytes and on many other cell types including endothelial, smooth muscle, kidney, liver, and neural cells. It mediates IL-8 release from bronchial epithelial cells. It also triggers an oxidative burst in macrophages and neutrophils, causing release of histamine in basophils and mast cells. C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion. Mouse C5a shares 60% and 82% sequence identity to human and rat C5a, respectively.
Alternative Names: Complement C5, Hemolytic Complement, C5, Hc

Molekulargewicht: 9 kDa

UniProt: P06684

Pathways: Komplementsystem, Carbohydrate Homeostasis