CPA1 Protein (AA 17-419) (His tag)

Produktnummer ABIN1096218

Dieses Recombinant CPA1-Protein wird in Human Cells produziert.

Kurzübersicht für CPA1 Protein (AA 17-419) (His tag) (ABIN1096218)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Human Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 17-419

Aufreinigungstag / Konjugat: Dieses CPA1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Carboxypeptidase A1/CPA1 (C-6His)

Sequenz: KEDFVGHQVL RISVADEAQV QKVKELEDLE HLQLDFWRGP AHPGSPIDVR VPFPSIQAVK IFLESHGISY ETMIEDVQSL LDEEQEQMFA FRSRARSTDT FNYATYHTLE EIYDFLDLLV AENPHLVSKI QIGNTYEGRP IYVLKFSTGG SKRPAIWIDT GIHSREWVTQ ASGVWFAKKI TQDYGQDAAF TAILDTLDIF LEIVTNPDGF AFTHSTNRMW RKTRSHTAGS LCIGVDPNRN WDAGFGLSGA SSNPCSETYR GKFANSEVEV KSIVDFVKDH GNIKAFISIH SYSQLLMYPY GYKTEPVPDQ DELDQLSKAA VTALASLYGT KFNYGSIIKA IYQASGSTID WTYSQGIKYS FTFELRDTGR YGFLLPASQI IPTAKETWLA LLTIMEHTLN HPYVDHHHHH H

Produktmerkmale:

Recombinant Human Carboxypeptidase A1/CPA1 (C-6His)

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150mm NaCl, pH 7.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Andere Bezeichnung: cpa1

Hintergrund: Recombinant Human Carboxypeptidase A1/CPA1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ala111-Tyr419) of Human CPA1 fused with a polyhistidine tag at the C-terminus.
Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.

Molekulargewicht: 46.57 kDa

UniProt: P15085