Annexin V Protein (DY-490)
(1 reference)Kurzübersicht für Annexin V Protein (DY-490) (ABIN1047953)
Target
Alle Annexin V (ANXA5) Proteine anzeigenProtein-Typ
Spezies
Wirt
-
-
Aufreinigungstag / Konjugat
- Dieses Annexin V Protein ist gelabelt mit DY-490.
-
Produktmerkmale
-
Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5 -
Benötigtes Material
-
Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml
-
-
Möchten Sie weitere Optionen für dieses Protein ?
!Entdecken Sie unsere vordefinierten maßgeschneiderten Proteine und Proteinservices!Ihr Projekt erfordert weitere Anpassungen? Kontaktieren Sie uns und entdecken Sie unsere maßgeschneiderten Proteinlösungen
-
Annexin A5 (ANXA5) (AA 2-320) protein
ANXA5 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Annexin A5 (ANXA5) protein (FITC)ANXA5 Spezies: Human Wirt: Bacteria Recombinant > 99 % (SDS-PAGE) FACS
Annexin A5 (ANXA5) (AA 1-319) protein (His-EGFP Tag)ANXA5 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Annexin A5 (ANXA5) (AA 1-320) protein (GST tag)ANXA5 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Annexin A5 (ANXA5) (AA 1-320) proteinANXA5 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
Annexin A5 (ANXA5) protein (FITC)ANXA5 Spezies: Säugetier Wirt: Escherichia coli (E. coli) BCA
Annexin A5 (ANXA5) proteinANXA5 Spezies: Säugetier Wirt: Escherichia coli (E. coli) BCA
Annexin A5 (ANXA5) protein (Pacific Blue)ANXA5 Spezies: Säugetier Wirt: Escherichia coli (E. coli) BCA
-
-
Applikationshinweise
- 5 l are suitable for one assay
-
Testdurchführung
-
Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour). -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Lagerung
- 4 °C
-
-
-
: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
-
: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." in: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
-
-
