Methyl-CpG Binding Domain Protein 1 (MBD1) Peptid
Kurzübersicht für Methyl-CpG Binding Domain Protein 1 (MBD1) Peptid (ABIN8105266)
Target
Quelle
Applikation
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Verwendungszweck
- MBD1 Peptide
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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This is a synthetic peptide designed for use in combination with anti-MBD1 antibody ( ARP40168_T100). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications. Please inquire for more details.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Add 100 µL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Buffer
- Lyophilized powder
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Lagerung
- -20 °C
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Informationen zur Lagerung
- For longer periods of storage, store at -20 °C. Avoid repeat freeze-thaw cycles.
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- MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))
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Hintergrund
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Background Information: MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
Alternative Symbols: RFT, PCM1, CXXC3
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Molekulargewicht
- 60kDa
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Gen-ID
- 4152
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NCBI Accession
- NP_056669
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UniProt
- Q9UIS9
Target
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