When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
CHMP5
(Charged Multivesicular Body Protein 5 (CHMP5))
Synonyme
SNF7DC2 Peptide, cgi-34 Peptide, hspc177 Peptide, pnas-2 Peptide, vps60 Peptide, chmp5 Peptide, zgc:64125 Peptide, C9orf83 Peptide, HSPC177 Peptide, PNAS-2 Peptide, Vps60 Peptide, 2210412K09Rik Peptide, AW545668 Peptide, RGD1305968 Peptide, charged multivesicular body protein 5 S homeolog Peptide, charged multivesicular body protein 5b Peptide, charged multivesicular body protein 5 Peptide, charged multivesicular body protein 5 L homeolog Peptide, chromatin modifying protein 5 Peptide, chmp5.S Peptide, chmp5b Peptide, CHMP5 Peptide, chmp5.L Peptide, chmp5 Peptide, Chmp5 Peptide