When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
HMGA1
(High Mobility Group AT-Hook 1 (HMGA1))
Synonyme
HMG-R Peptide, HMGA1A Peptide, HMGIY Peptide, Hmgi Peptide, Hmgiy Peptide, hmga1 Peptide, hmgiy Peptide, wu:fb12b06 Peptide, wu:fb18f11 Peptide, wu:fc19f01 Peptide, wu:fi38b05 Peptide, zgc:85677 Peptide, AL023995 Peptide, Hmga1a Peptide, Hmga1b Peptide, Hmgy Peptide, HMG-I(Y) Peptide, HMG-Y Peptide, HMGA1 Peptide, zgc:153948 Peptide, high mobility group AT-hook 1 Peptide, high mobility group protein HMG-I/HMG-Y Peptide, high mobility group AT-hook 1a Peptide, high mobility group AT-hook 1b Peptide, HMGA1 Peptide, Hmga1 Peptide, TSC_c24220 Peptide, hmga1a Peptide, LOC705105 Peptide, hmga1b Peptide