When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
Splicing factor U2AF 26 kDa subunit (U2AF1L4)
Synonyme
U2 small nuclear RNA auxiliary factor 1-like protein 3 Peptide, U2AF1-RS3 Peptide, U2AF1-like 4 Peptide, U2AF1-like protein 3 Peptide, U2AF1L3 Peptide, U2AF1L3V1 Peptide, U2AF1RS3 Peptide, U2af26 Peptide, AA407033 Peptide, AF419339 Peptide, AI451269 Peptide, AW553050 Peptide, TMEM149 Peptide, U2 small nuclear RNA auxiliary factor 1 like 4 Peptide, U2 small nuclear RNA auxiliary factor 1-like 4 Peptide, U2AF1L4 Peptide, U2af1l4 Peptide