Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

C3 ELISA Kit

C3 Reaktivität: Ratte Colorimetric Sandwich ELISA
Produktnummer ABIN956041
  • Target Alle C3 ELISA Kits anzeigen
    C3 (Complement Component 3 (C3))
    Reaktivität
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    The Rat Complement C3 ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative determination of Complement C3 in rat biological samples.
    Analytische Methode
    Quantitative
    Produktmerkmale
    In this assay the Complement C3 present in the sample reacts with the anti-Complement C3 antibody, which has been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Complement C3 antibody conjugated with horseradish peroxidase (HRP) is added. This HRP-conjugated antibody forms a complex with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme is proportional to the concentration of Complement C3 in the sample tested, thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the calibration curve constructed from the calibrators and corrected for sample dilution.
    Bestandteile
    1. Diluent Concentrate: One bottle containing 50 mL of a 5X concentrated diluent running buffer.
    2. Wash Solution Concentrate: One bottle containing 50 mL of a 20X concentrated wash solution.
    3. Enzyme-Antibody Conjugate Concentrate: One vial containing 150 µL of a 100X concentrated affinity-purified anti-rat Complement C3 antibody conjugated with HRP in stabilizing buffer.
    4. TMB Substrate Solution: One vial containing 12 mL of TMB and hydrogen peroxide in citric acid buffer at pH 3.3.
    5. Stop Solution: One vial containing 12 mL of 0.3 M sulfuric acid. WARNING: Avoid contact with skin.
    6. Microtiter Plate: Twelve removable eight-well strips in well holder frame. Wells are coated with affinity-purified anti-rat Complement C3.
    7. Rat Complement C3 Calibrator: One vial containing a lyophilized Rat Complement C3 Calibrator.
    8. Positive Control: One vial containing 50 µL of serum with 0.1% sodium azide. See the Control Certificate for the concentration.
    Benötigtes Material
    Precision pipettes (2 µL to 200 µL) for making and dispensing dilutions
    Test tubes
    Microplate washer/aspirator
    Distilled or de-ionized H2O Microplate reader
    Assorted glassware for the preparation of reagents and buffer solutions
    Timer
    Vortex mixer
    Featured
    Zu unserem meistverkauften C3 ELISA Kit
    Top Product
    Discover our top product C3 ELISA Kit
  • Plattentyp
    Pre-coated
    Aufbereitung der Reagenzien
    1. Diluent Concentrate: The Diluent solution supplied is a 5X concentrate and must be diluted 1:5 with distilled or de-ionized water.
      2. Wash Solution Concentrate: The Wash Solution supplied is a 20X concentrate and must be diluted 1:20 with distilled or de-ionized water. Crystal formation in the concentrate is not uncommon when storage temperatures are low. Warming of the concentrate to 30
      35°C before dilution can dissolve crystals.
      3. Enzyme-Antibody Conjugate Concentrate: Calculate the required amount of working conjugate solution for each microtiter plate test strip by adding 10 µL Enzyme-Antibody Conjugate to 990 µL of 1X Diluent for each test strip to be used for testing. Mix uniformly, but gently. Avoid foaming.
      4. TMB Substrate Solution: Ready to use as supplied.
      5. Stop Solution: Ready to use as supplied.
      6. Microtiter Plate: Ready to use as supplied. Unseal Microtiter Pouch and remove plate from pouch. Remove all strips and wells that will not be used in the assay and place back in pouch and re-seal along with desiccant.
      7. Rat Complement C3 Calibrator: Add 1.0 mL of distilled or de-ionized water to the lyophilized Rat Complement C3 Calibrator and mix gently until dissolved. The calibrator is now at a concentration of 5.76 µg/mL (the reconstituted calibrator should be aliquoted and frozen if future use is intended). Rat Complement C3 Calibrators need to be prepared immediately prior to use. Mix well between each step. Avoid foaming.
      8. Positive Control: The concentration and recommended dilution are provided on the Control Certificate. Before use, briefly centrifuge the Positive Control to allow all of the liquid to collect in the bottom of the vial.
    Ergebnisberechnung
    1. Subtract the average background value from the test values for each sample.
      2. Using the results observed for the calibrators construct a calibration curve. The appropriate curve fit is that of a four- parameter logistics curve, although a second order polynomial (quadratic) or other curve fits may also be used.
      3. Interpolate test sample values from calibration curve. Correct for sample dilution factor to arrive at Complement C3 concentration in original sample.

      1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the information contained in the package insert instructions and with adherence to good laboratory practice.
      2. Factors that might affect the performance of the assay include proper instrument function, cleanliness of glassware, quality of distilled or de-ionized water, washing thoroughly and accuracy of reagent and sample pipettings, washing technique, incubation time or temperature.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    1. Complete Kit: The expiration date for the kit is stated on the outer label. The recommended storage temperature is 4°C. Note: See long term storage recommendations below for the Rat Complement C3 Calibrator and Positive Control.
    2. Diluent: The 5X Diluent Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4°C.
    3. Wash Solution: The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (RT, 16 - 25°C) or at 4°C.
    4. Enzyme-Antibody Conjugate: Undiluted anti-Complement C3-HRP conjugate should be stored at 4°C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
    5. TMB Substrate Solution: The TMB Substrate Solution should be stored at 4°C and is stable until the expiration date.
    6. Stop Solution: The Stop Solution should be stored at 4°C and is stable until the expiration date.
    7. Microtiter Plate: Anti-rat Complement C3 coated wells are stable until the expiration date, and should be stored at 4°C in the sealed foil pouch with desiccant pack.
    8. Rat Complement C3 Calibrator: The lyophilized Rat Complement C3 Calibrator should be stored at 4°C or frozen until reconstituted. The reconstituted calibrator should be aliquoted and stored frozen. Avoid multiple freeze/thaw cycles. The working calibrator solutions should be prepared immediately prior to use and are stable for up to 8 hours.
    9. Positive Control: For storage longer than 7 days keep frozen until the expiration date. Storage less than 7 days can be at 4°C. Avoid multiple freeze/thaw cycles.
    Haltbarkeit
    The expiry date is stated on the label.
  • Target Alle C3 ELISA Kits anzeigen
    C3 (Complement Component 3 (C3))
    Andere Bezeichnung
    Complement C3 (C3 Produkte)
    Synonyme
    AHUS5 ELISA Kit, ARMD9 ELISA Kit, ASP ELISA Kit, C3a ELISA Kit, C3b ELISA Kit, CPAMD1 ELISA Kit, AI255234 ELISA Kit, HSE-MSF ELISA Kit, Plp ELISA Kit, C3 ELISA Kit, C3-1 ELISA Kit, sb:cb26 ELISA Kit, si:dkey-76b14.4 ELISA Kit, wu:fi34b03 ELISA Kit, wu:fj86e11 ELISA Kit, C3d ELISA Kit, asp ELISA Kit, cpamd1 ELISA Kit, c3 ELISA Kit, complement C3 ELISA Kit, complement component 3 ELISA Kit, complement component c3a, duplicate 1 ELISA Kit, complement component 3 L homeolog ELISA Kit, complement component C3 ELISA Kit, complement C3-like ELISA Kit, C3 ELISA Kit, c3a.1 ELISA Kit, c3.L ELISA Kit, LOC443475 ELISA Kit, c3 ELISA Kit, c3-1 ELISA Kit, LOC100060539 ELISA Kit
    Hintergrund
    A number of serum proteins participate in acute inflammatory reactions. These include the complement, coagulation and kinin systems as well as a number of other proteins, known as acute phase proteins, that regulate acute inflammation. The complement system is a complex set of up to 20 different serum proteins. The most abundant and pivotal of the complement components is Complement C3, which has a molecular weight of about 187 kDa and consists of an alpha and beta chain
    Pathways
    Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
Sie sind hier:
Kundenservice