C3a ELISA Kit

Produktnummer ABIN955830

Das Ratte C3a ELISA Kit (ABIN955830) ist ein Colorimetric ELISA Kit zur Detektion von Ratte C3a.

Kurzübersicht für C3a ELISA Kit (ABIN955830)

Target: C3a (Complement Fragment 3a (C3a))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625-40 ng/mL

Applikation: ELISA

Produktdetails C3a ELISA Kit

Untere Nachweisgrenze: 0.625 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of rat C3a in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of rat C3a. No significant cross- reactivity or interference between rat C3a and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between rat C3a and all the analogues, therefore, cross reaction may still exist.

Sensitivität: The minimum detectable dose of rat C3 a is typically less than 0.225 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.

Produktmerkmale: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3a. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of C3a in the samples is then determined by comparing the O.D. of the samples to the calibration curve.

Bestandteile: Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x).

Benötigtes Material: 1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Kommentare:

The calibration curve concentrations used for the ELISA's were 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL.

Plattentyp: Pre-coated

Ergebnisberechnung:

Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with C3a concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.

Haltbarkeit: The expiry date is stated on the label.

Details zu C3a

Target: C3a (Complement Fragment 3a (C3a))

Andere Bezeichnung: Complement Component 3a (C3a)

Pathways: Komplementsystem