alpha Fetoprotein ELISA Kit

Produktnummer ABIN930400

Produktdetails alpha Fetoprotein ELISA Kit

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of AFP in the research laboratory
Alternative Names: AFP ELISA kit, alpha-Fetoprotein ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Plattentyp: Pre-coated

Testdurchführung:

The AFP is a direct solid phase sandwich ELISA method. The samples and diluted antiAFPHRP conjugate are added to the wells coated with MAb to beta subunit. AFP in the patient's serum binds to antiAFP MAb on the well and the antiAFP second antibody then binds to AFP. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of AFP in the samples. A standard curve is prepared relating color intensity to the concentration of the AFP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: AFP (AFP Produkte)

Hintergrund: Alpha fetoprotein (AFP) is a glycoprotein with a molecular weigh of approximately 70,000 Daltons. AFP is normally produced during fetal and neonatal development by the liver, yolksac, and in small concentrations by the gastrointestinal tract. After birth, serum AFP concentrations decrease rapidly, and by the second year of life and thereafter only trace amounts are normally detected in serum. Elevation of serum AFP to abnormally high values occurs in several malignant diseases, most notably non-seminomatous testicular cancer and primary hepatocellular carcinoma. In the case of non-seminomatous testicular cancer, a direct relationship has been observed between the incidence of elevated AFP levels and the stage of disease. Elevated AFP levels have also been observed in patients diagnosed with seminoma with non-seminomatous elements, but not in patients with pure seminoma. In addition, elevated serum AFP concentrations have been measured in patients with other noncancerous diseases, including ataxia telangiectasia, hereditary tyrosinemia, neonatal hyperbilirubinemia, acute viral hepatitis, chronic active hepatitis, and cirrhosis. Elevated serum AFP concentrations are also observed in pregnant women. Therefore, AFP measurements are not recommended for use as a screening procedure to detect the presence of cancer in the general population.
Synonyms: AFP ELISA kit, alpha-Fetoprotein ELISA kit.

Pathways: C21-Steroid Hormone Metabolic Process