RBP4 ELISA Kit

Produktnummer ABIN921108

Dieses Maus RBP4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus RBP4 zu quantifizieren.

Kurzübersicht für RBP4 ELISA Kit (ABIN921108)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Bindungsspezifität: AA 19-201

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312-20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Produktdetails RBP4 ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse RBP4

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: E19-L201

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E19-L201

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.

Plattentyp: Pre-coated

Protokoll: mouse RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RBP4 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse RBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin , EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse RBP4 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 1.34, Standard deviation: 0.06, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 4.6, Standard deviation: 0.348, CV(%): 5.7
• Sample 3: n=16, Mean(ng/ml): 10.12, Standard deviation: 0.617, CV(%): 6.1,
• Sample 1: n=24, Mean(ng/ml): 1.65, Standard deviation: 0.102, CV(%): 6.2
• Sample 2: n=24, Mean(ng/ml): 4.34, Standard deviation: 0.304, CV(%): 7
• Sample 3: n=24, Mean(ng/ml): 9.86, Standard deviation: 0.76, CV(%): 7.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Andere Bezeichnung: RBP4

Hintergrund:

Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37-K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Synonyms: Retinol-binding protein 4 ,Rbp4 ,

Full Gene Name: retinol binding protein 4, plasma

Gen-ID: 19662

UniProt: H7BWY6

Pathways: Regulatorische RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response