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RBP4 ELISA Kit

RBP4 Reaktivität: Human AA 19-201 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Produktnummer ABIN921107
  • Target Alle RBP4 ELISA Kits anzeigen
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    Bindungsspezifität
    AA 19-201
    Reaktivität
    • 8
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312-20000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human RBP4
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: E19-L201
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E19-L201
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.

    Plattentyp
    Pre-coated
    Protokoll
    human RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human RBP4 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human RBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human RBP4 standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 2.8, Standard deviation: 0.174, CV(%): 6.2
    • Sample 2: n=16, Mean(ng/ml): 5, Standard deviation: 0.295, CV(%): 5.9
    • Sample 3: n=16, Mean(ng/ml): 9.43, Standard deviation: 0.434, CV(%): 4.6,
    • Sample 1: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.255, CV(%): 7.5
    • Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.422, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 11.5, Standard deviation: 0.874, CV(%): 7.6
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Ding, Luo, Yu, Zhou, Wu: "Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment." in: Molecular vision, Vol. 24, pp. 252-260, (2018) (PubMed).

    Farahbakhsh-Farsi, Djazayery, Eshraghian, Koohdani, Zarei, Javanbakht, Derakhshanian, Djalali: "Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients." in: Iranian journal of public health, Vol. 45, Issue 2, pp. 179-85, (2016) (PubMed).

  • Target Alle RBP4 ELISA Kits anzeigen
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    Andere Bezeichnung
    RBP4 (RBP4 Produkte)
    Synonyme
    RDCCAS ELISA Kit, RBP ELISA Kit, RNBP ELISA Kit, rbp4 ELISA Kit, MGC54038 ELISA Kit, srbp ELISA Kit, PRBP ELISA Kit, RBPA ELISA Kit, Rbp-4 ELISA Kit, fb23c12 ELISA Kit, rbp ELISA Kit, wu:fb23c12 ELISA Kit, wu:fb58d04 ELISA Kit, wu:fb72b04 ELISA Kit, zgc:86911 ELISA Kit, retinol binding protein 4 ELISA Kit, renin binding protein ELISA Kit, retinol binding protein 4 S homeolog ELISA Kit, retinol binding protein 4 A, plasma ELISA Kit, retinol binding protein 4, plasma ELISA Kit, retinol binding protein 4 L homeolog ELISA Kit, RBP4 ELISA Kit, RENBP ELISA Kit, rbp4.S ELISA Kit, rbp4 ELISA Kit, Rbp4 ELISA Kit, RBP4A ELISA Kit, rbp4.L ELISA Kit
    Hintergrund

    Protein Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

    Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

    Synonyms: Retinol-binding protein 4,Plasma retinol-binding protein,PRBP,RBP,Plasma retinol-binding protein(1-182),Plasma retinol-binding protein(1-181),Plasma retinol-binding protein(1-179),Plasma retinol-binding protein(1-176),RBP4,PRO2222,

    Full Gene Name: Retinol-binding protein 4

    Cellular Localisation: Secreted.
    Gen-ID
    5950
    UniProt
    P02753
    Pathways
    Regulatorische RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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