IL-7 ELISA Kit

Produktnummer ABIN921098

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-7 in Cell Culture Supernatant, Serum und Plasma (EDTA). Es ist 1 Publikation verfügbar.

Kurzübersicht für IL-7 ELISA Kit (ABIN921098)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Bindungsspezifität: AA 26-177

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (EDTA)

Produktdetails IL-7 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-7

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: D26-H177

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: D26-H177

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli, D26-H177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-7 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-7 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 54.5, Standard deviation: 3.38, CV(%): 6.2
• Sample 2: n=16, Mean(pg/ml): 368, Standard deviation: 16.56, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 759, Standard deviation: 36.67, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 55.9, Standard deviation: 3.86, CV(%): 6.9
• Sample 2: n=24, Mean(pg/ml): 396, Standard deviation: 27.72, CV(%): 7
• Sample 3: n=24, Mean(pg/ml): 737, Standard deviation: 41.3, CV(%): 5.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-7 ELISA Kit (ABIN921098)

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL7

Hintergrund:

Protein Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. .

Background: IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.

Synonyms: Interleukin-7 ,IL-7 ,

Full Gene Name: Interleukin-7

Cellular Localisation: Secreted.

Gen-ID: 3574

UniProt: A8K673

Pathways: JAK-STAT Signalweg