CD80 ELISA Kit

Produktnummer ABIN921092

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CD80. Es sind 2 Publikationen verfügbar.

Kurzübersicht für CD80 ELISA Kit (ABIN921092)

Target: CD80

Bindungsspezifität: AA 37-245

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails CD80 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse B7-1/CD80

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: D37-K245

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D37-K245

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Tissue Specificity: Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies.

Plattentyp: Pre-coated

Protokoll: mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD80 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 0.28, Standard deviation: 0.015, CV(%): 5.2
• Sample 2: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.111, CV(%): 7.1
• Sample 3: n=16, Mean(ng/ml): 2.31, Standard deviation: 0.113, CV(%): 4.9,
• Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.016, CV(%): 6.2
• Sample 2: n=24, Mean(ng/ml): 1.72, Standard deviation: 0.138, CV(%): 8.0
• Sample 3: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.134, CV(%): 5.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CD80 ELISA Kit (ABIN921092)

Veinalde, Grossardt, Hartmann, Bourgeois-Daigneault, Bell, Jäger, von Kalle, Ungerechts, Engeland: "Oncolytic measles virus encoding interleukin-12 mediates potent antitumor effects through T cell activation." in: Oncoimmunology, Vol. 6, Issue 4, pp. e1285992, (2017) (PubMed).

Du, Dong, Zhao, Fu, Wang, Chen, Ou, Li, Sun, Tang, Song: "Immunostimulatory and anti-neoplasm effects of a novel palindrome CpG oligodeoxynucleotide in mice." in: Acta pharmacologica Sinica, Vol. 33, Issue 8, pp. 1047-54, (2012) (PubMed).

Details zu CD80

Target: CD80

Andere Bezeichnung: CD80

Hintergrund:

Protein Function: Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Background: The protein CD80(Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86(601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1, D37-K245, which is composed of two single chains acids with the dipolymer.

Synonyms: T-lymphocyte activation antigen CD80,Activation B7-1 antigen,B7,CD80,Cd80,B7,

Full Gene Name: T-lymphocyte activation antigen CD80

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 12519

UniProt: Q00609

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints