Galectin 3 ELISA Kit

Produktnummer ABIN8126255

Schwein Galectin 3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Schwein Galectin 3.

Kurzübersicht für Galectin 3 ELISA Kit (ABIN8126255)

Target: Galectin 3 (LGALS3)

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Galectin 3 ELISA Kit

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Porcine Gal 3 (Galectin 3) concentrations in serum, plasma. Detection Range: 0.156-10 ng/mL Sensitivity: <0.094 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GAL3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GAL3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GAL3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GAL3 can be calculated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu Galectin 3

Target: Galectin 3 (LGALS3)

Andere Bezeichnung: GAL3 (Galectin 3)

Pathways: RTK Signalweg