IL-4 ELISA Kit

Produktnummer ABIN8126250

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus IL-4.

Kurzübersicht für IL-4 ELISA Kit (ABIN8126250)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.625 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-4 ELISA Kit

Untere Nachweisgrenze: 15.625 pg/mL

Verwendungszweck: This assay employs an antibody specific for Anti-mouse IL-4 coated on a 96-well plate.

Analytische Methode: Quantitative

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma.  Detection Range: 15.625-1000pg/mL Sensitivity: <9.375pg/mL  

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-mouse IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL-4 (Interleukin 4)

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation