IL-13 ELISA Kit

Produktnummer ABIN8126245

Das Maus IL-13 ELISA Kit (ABIN8126245) ist ein Colorimetric ELISA Kit zur Detektion von Maus IL-13.

Kurzübersicht für IL-13 ELISA Kit (ABIN8126245)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-13 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Sensitivität: 18.75 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma.  Detection Range: 31.25 - 2000 pg/mL Sensitivity: < 18.75 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-13 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-13 can be calculated.  

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: IL-13 (Interleukin 13)

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport