IL-10 ELISA Kit

Produktnummer ABIN8126244

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus IL-10.

Kurzübersicht für IL-10 ELISA Kit (ABIN8126244)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-10 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate.

Analytische Methode: Quantitative

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma.  Detection Range: 15.6 - 1000 pg/mL Sensitivity: <9.4pg / ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-Mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: IL-10 (Interleukin 10)

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints