GM-CSF ELISA Kit

Produktnummer ABIN8126236

Das Human GM-CSF ELISA Kit (ABIN8126236) ist ein Colorimetric ELISA Kit zur Detektion von Human GM-CSF.

Kurzübersicht für GM-CSF ELISA Kit (ABIN8126236)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.9 pg/mL - 250 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails GM-CSF ELISA Kit

Untere Nachweisgrenze: 3.9 pg/mL

Verwendungszweck: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytische Methode: Quantitative

Sensitivität: 2.34 pg/mL

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Human GMCSF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: < 2.34 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human GMCSF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human GMCSF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GMCSF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human GMCSF can be calculated using the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: GMCSF (Granulocyte Macrophage Colony Stimulating Factor)

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin