EPO ELISA Kit

Produktnummer ABIN8090119

Human EPO ELISA Kit, Colorimetric Assay für die Quantifizierung von Human EPO.

Kurzübersicht für EPO ELISA Kit (ABIN8090119)

Target: EPO (Erythropoietin (EPO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 mIU/mL - 200 mIU/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Güteklasse: Quick

Produktdetails EPO ELISA Kit

Untere Nachweisgrenze: 3.12 mIU/mL

Verwendungszweck: Human EPO (Erythropoietin) QuickTest ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with EPO , no obvious cross reaction with other analogues.

Sensitivität: 1.875 mIU/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Cap/Det Ab (Ready to use
• blue)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu EPO

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: EPO

Substanzklasse: Hormone

Hintergrund: Erythropoietin, EPO, Epoetin Alfa, erythropoietin alpha

UniProt: P01588

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity