AMPK alpha ELISA Kit

Produktnummer ABIN8087108

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus, Human und Ratte AMPK alpha.

Kurzübersicht für AMPK alpha ELISA Kit (ABIN8087108)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Bindungsspezifität: pThr172

Reaktivität: Maus, Human, Ratte

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails AMPK alpha ELISA Kit

Verwendungszweck: Phospho-AMPKα (Thr172) Colorimetric Cell-Based ELISA Kit for detecting Phospho-AMPKα (Thr172) in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Andere Bezeichnung: AMPKalpha

Hintergrund: Gene Aliases: 5'-AMP-activated protein kinase, catalytic alpha-1 chain, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1, acetyl-CoA carboxylase kinase, hormone-sensitive lipase kinase, kinase AMPK-alpha1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, PRKAA2, AMPK, AMPK2

Gen-ID: 5562

UniProt: Q13131

Pathways: Warburg Effekt