SMAD2 ELISA Kit

Produktnummer ABIN8087059

Maus SMAD2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus SMAD2.

Kurzübersicht für SMAD2 ELISA Kit (ABIN8087059)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails SMAD2 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Mouse Mothers Against Decapentaplegic Homolog 2 (Smad2) ELISA Kit for detecting Smad2 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 28.4 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Andere Bezeichnung: Mothers Against Decapentaplegic Homolog 2

Hintergrund: Gene Aliases: JV18, JV18-1, MADH2, MADR2, HMAD-2, HSMAD2, Mad-related protein 2

Gen-ID: 17126

UniProt: Q62432

Pathways: Zellzyklus, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus