SFRP1 ELISA Kit

Produktnummer ABIN8086608

Das Human SFRP1 ELISA Kit (ABIN8086608) ist ein Colorimetric ELISA Kit zur Detektion von Human SFRP1.

Kurzübersicht für SFRP1 ELISA Kit (ABIN8086608)

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails SFRP1 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Human Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit for detecting SFRP1 in serum, plasma, tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.057 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Andere Bezeichnung: Secreted Frizzled Related Protein 1

Hintergrund: Gene Aliases: FRP1, FRP, FRP-1, FrzA, SARP2, Secreted apoptosis-related protein 2

Gen-ID: 6422

UniProt: Q8N474

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation