PPP2CA ELISA Kit

Produktnummer ABIN8085923

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human PPP2CA.

Kurzübersicht für PPP2CA ELISA Kit (ABIN8085923)

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails PPP2CA ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Human Protein Phosphatase 2, Catalytic Subunit Alpha Isoform (PPP2Ca) ELISA Kit for detecting PPP2Ca in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 30 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Andere Bezeichnung: Protein Phosphatase 2, Catalytic Subunit Alpha Isoform

Hintergrund: Gene Aliases: PP2CA, PP2Ac, RP-C, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Gen-ID: 5515

UniProt: P67775

Pathways: PI3K-Akt Signalweg, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades