PRKAA1 ELISA Kit

Produktnummer ABIN8085887

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Ratte PRKAA1.

Kurzübersicht für PRKAA1 ELISA Kit (ABIN8085887)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails PRKAA1 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Rat Protein Kinase, AMP Activated Alpha 1 (AMPK Alpha 1) ELISA Kit for detecting AMPK Alpha 1 in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 0.55 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Andere Bezeichnung: Protein Kinase, AMP Activated Alpha 1

Hintergrund: Gene Aliases: PRKAa1, AMPK, AMPKa1, PKA, ACACA kinase, 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1, Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase

Gen-ID: 65248

UniProt: P54645

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt