PKC epsilon CLIA Kit
Kurzübersicht für PKC epsilon CLIA Kit (ABIN8085863)
Target
Alle PKC epsilon (PRKCE) CLIA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 0.14 ng/mL
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Verwendungszweck
- Human Protein Kinase C Epsilon (PKCe) CLIA Kit for detecting PKCe in tissue homogenates
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Analytische Methode
- Quantitative
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Sensitivität
- 0.07 ng/mL
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Bestandteile
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Benötigtes Material
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Probenmenge
- 100 μL
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Testdauer
- 3 h
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Plattentyp
- Pre-coated
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Testpräzision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C for one month, Long term: see manual.
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Haltbarkeit
- 12 months
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- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
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Andere Bezeichnung
- Protein Kinase C Epsilon
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Hintergrund
- Gene Aliases: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon
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Gen-ID
- 5581
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UniProt
- Q02156
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Pathways
- T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
Target Alle PKC epsilon (PRKCE) CLIA Kits anzeigen
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