PFKM ELISA Kit

Produktnummer ABIN8085130

Ratte PFKM ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte PFKM.

Kurzübersicht für PFKM ELISA Kit (ABIN8085130)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Produktdetails PFKM ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: Rat Phosphofructokinase, Muscle (PFKM) ELISA Kit for detecting PFKM in tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.112 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Andere Bezeichnung: Phosphofructokinase, Muscle

Hintergrund: Gene Aliases: PFK1, PFKX, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, 6-Phosphofructokinase Polypeptide X, Fructose-6-Phosphate 1-Phosphotransferase

Gen-ID: 65152

UniProt: P47858

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effekt