HMGB1 ELISA Kit
Kurzübersicht für HMGB1 ELISA Kit (ABIN8080631)
Target
Alle HMGB1 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 31.2 pg/mL
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Verwendungszweck
- Rat High Mobility Group Protein 1 (HMGB1) ELISA Kit for detecting HMGB1 in serum, plasma, tissue homogenates
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Analytische Methode
- Quantitative
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Sensitivität
- 12.2 pg/mL
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Bestandteile
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Benötigtes Material
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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High Mobility Group Box 1 (HMGB1) ELISA Kit
HMGB1 Reaktivität: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Maus Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Maus Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Ratte Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Hund Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Hund Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Probenmenge
- 100 μL
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Testdauer
- 3 h
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Plattentyp
- Pre-coated
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Testpräzision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C for one month, Long term: see manual.
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Haltbarkeit
- 12 months
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Andere Bezeichnung
- High Mobility Group Protein 1
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Hintergrund
- Gene Aliases: HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein
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Gen-ID
- 25459
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UniProt
- P63159
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Pathways
- p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target Alle HMGB1 ELISA Kits anzeigen
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