GRP94 ELISA Kit

Produktnummer ABIN8080442

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus GRP94.

Kurzübersicht für GRP94 ELISA Kit (ABIN8080442)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails GRP94 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Mouse Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit for detecting HSP90b1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 0.058 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: Heat Shock Protein 90kDa Beta 1

Hintergrund: Gene Aliases: HSP90-B1, ECGP, GP96, GRP94, TRA1, Glycoprotein 96, Tumor Rejection Antigen (gp96) 1,94 kDa glucose-regulated protein, Endoplasmin

Gen-ID: 22027

UniProt: P08113

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades