GADD45A ELISA Kit

Produktnummer ABIN8080153

Ratte GADD45A ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte GADD45A.

Kurzübersicht für GADD45A ELISA Kit (ABIN8080153)

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails GADD45A ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: Rat Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) ELISA Kit for detecting GADD45a in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 0.056 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu GADD45A

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Andere Bezeichnung: Growth Arrest And DNA Damage Inducible Protein Alpha

Hintergrund: Gene Aliases: DDIT1, GADD45, DNA damage-inducible transcript 1 protein

UniProt: P48317

Pathways: p53 Signalweg, Zellzyklus