Cholecystokinin ELISA Kit

Produktnummer ABIN8076743

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Hund Cholecystokinin.

Kurzübersicht für Cholecystokinin ELISA Kit (ABIN8076743)

Target: Cholecystokinin (CCK)

Reaktivität: Hund

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 12.35 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails Cholecystokinin ELISA Kit

Untere Nachweisgrenze: 12.35 pg/mL

Verwendungszweck: Dog Cholecystokinin (CCK) ELISA Kit for detecting CCK in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 5.25 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu Cholecystokinin

Target: Cholecystokinin (CCK)

Andere Bezeichnung: Cholecystokinin

Hintergrund: Gene Aliases: CCK-PZ

UniProt: G1K283

Pathways: T-Zell Rezeptor Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Feeding Behaviour