Angiotensin I Converting Enzyme 1 CLIA Kit

Produktnummer ABIN8074595

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Maus Angiotensin I Converting Enzyme 1.

Kurzübersicht für Angiotensin I Converting Enzyme 1 CLIA Kit (ABIN8074595)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 54.9 pg/mL - 40000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Produktdetails Angiotensin I Converting Enzyme 1 CLIA Kit

Untere Nachweisgrenze: 54.9 pg/mL

Verwendungszweck: Mouse Angiotensin I Converting Enzyme (ACE) CLIA Kit for detecting ACE in serum, plasma, tissue homogenates, cerebrospinal fluid, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 21.5 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Andere Bezeichnung: Angiotensin I Converting Enzyme

Hintergrund: Gene Aliases: CD143, ACE1, DCP1, ACEI, ACE-I, Kininase II, Angiotensin-Converting Enzyme, Peptidyl-Dipeptidase A, Dipeptidyl Carboxypeptidase 1, Angiotensin-converting enzyme, soluble form

Gen-ID: 11421

UniProt: P09470

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration