ATF4 ELISA Kit

Produktnummer ABIN8073973

Ratte ATF4 ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte ATF4.

Kurzübersicht für ATF4 ELISA Kit (ABIN8073973)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails ATF4 ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Rat Activating Transcription Factor 4 (ATF4) ELISA Kit for detecting ATF4 in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 29 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Andere Bezeichnung: Activating Transcription Factor 4

Hintergrund: Gene Aliases: TAXREB67, TXREB, CREB-2, DNA-binding protein TAXREB67, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67

Gen-ID: 79255

UniProt: Q9ES19

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response