14-3-3 zeta ELISA Kit

Produktnummer ABIN8073777

Das Human, Ratte und Maus 14-3-3 zeta ELISA Kit (ABIN8073777) ist ein Colorimetric ELISA Kit zur Detektion von Human, Ratte und Maus 14-3-3 zeta.

Kurzübersicht für 14-3-3 zeta ELISA Kit (ABIN8073777)

Target: 14-3-3 zeta (YWHAZ)

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails 14-3-3 zeta ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Human/Mouse/Rat Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) ELISA Kit for detecting YWHAz in tissue homogenates, cell lysates

Analytische Methode: Quantitative

Sensitivität: 12.2 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Andere Bezeichnung: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta

Hintergrund: Gene Aliases: KCIP-1, 14-3-3-zeta, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1

Gen-ID: 7534

UniProt: P63104

Pathways: Apoptose, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location