PRKAA1 ELISA Kit

Produktnummer ABIN8070559

Das Human, Maus und Ratte PRKAA1 ELISA Kit (ABIN8070559) ist ein Colorimetric ELISA Kit zur Detektion von Human, Maus und Ratte PRKAA1.

Kurzübersicht für PRKAA1 ELISA Kit (ABIN8070559)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Bindungsspezifität: pThr183, total

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails PRKAA1 ELISA Kit

Verwendungszweck: Phospho-AMPKa1 (T183) and Total AMPKa1 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human, mouse, rat cell lysates.

Analytische Methode: Semi-Quantitative

Spezifität: The antibody pair provided in this kit recognizes human, mouse and rat AMPK subunit alpha-1 phosphorylated at site Threonine-183.

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Positive Control
• Anti-Phospho Detection Antibody
• Anti-Pan Detection Antibody (*not included in phospho-only version)
• HRP-Conjugated Secondary Antibody
• HRP-Streptavidin (*not included in phospho-only version)
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Details zu PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Andere Bezeichnung: AMPKa1

Hintergrund:

Gene Symbols: PRKAA1, AMPK1

Protein Name (Synonyms): 5'-AMP-activated protein kinase catalytic subunit alpha-1 (AMPK subunit alpha-1), Acetyl-CoA carboxylase kinase (ACACA kinase), Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase), Tau-protein kinase PRKAA1

Gen-ID: 556210578765248

UniProt: Q13131, Q5EG47, P54645

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt